SUPERFAMILY 1.75 HMM library and genome assignments server

DnaJ/Hsp40 cysteine-rich domain superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Small proteins [ 56992] (90)
Fold:   DnaJ/Hsp40 cysteine-rich domain [ 57937]
Superfamily:   DnaJ/Hsp40 cysteine-rich domain [ 57938]
Families:   DnaJ/Hsp40 cysteine-rich domain [ 57939] (2)


Superfamily statistics
Genomes (3,158) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 7,356 25,611 2
Proteins 7,337 25,580 2


Functional annotation
General category Information
Detailed category Transcription

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) protein metabolic process 0.000227 Least Informative Direct
Biological Process (BP) cellular macromolecule metabolic process 0.00009496 Least Informative Direct
Biological Process (BP) regulation of metabolic process 0.3569 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 0.1787 Least Informative Inherited
Biological Process (BP) positive regulation of biological process 0.4472 Least Informative Inherited
Biological Process (BP) localization 0.005291 Least Informative Inherited
Biological Process (BP) multicellular organismal process 0.4085 Least Informative Inherited
Biological Process (BP) single-organism cellular process 1 Least Informative Inherited
Biological Process (BP) response to stimulus 0.08222 Least Informative Inherited
Biological Process (BP) regulation of cellular process 0.7611 Least Informative Inherited
Biological Process (BP) regulation of catalytic activity 0.0001452 Moderately Informative Direct
Biological Process (BP) regulation of phosphorus metabolic process 0.000003708 Moderately Informative Direct
Biological Process (BP) regulation of signal transduction 0.2 Moderately Informative Inherited
Biological Process (BP) cellular localization 0.09929 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 0.244 Moderately Informative Inherited
Biological Process (BP) macromolecule localization 0.04152 Moderately Informative Inherited
Biological Process (BP) regulation of nucleobase-containing compound metabolic process 1 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.8061 Moderately Informative Inherited
Biological Process (BP) negative regulation of metabolic process 0.7139 Moderately Informative Inherited
Biological Process (BP) signal transduction 0.2325 Moderately Informative Inherited
Biological Process (BP) multi-organism process 0.1045 Moderately Informative Inherited
Biological Process (BP) reproductive process 0.007582 Moderately Informative Inherited
Biological Process (BP) regulation of protein metabolic process 0.1059 Moderately Informative Inherited
Biological Process (BP) regulation of nucleoside metabolic process 0.0001647 Informative Direct
Biological Process (BP) sexual reproduction 0.0006028 Informative Direct
Biological Process (BP) negative regulation of cellular protein metabolic process 0.0009494 Informative Direct
Biological Process (BP) single-organism membrane organization 0.0007008 Informative Direct
Biological Process (BP) negative regulation of cell death 0.00006059 Informative Direct
Biological Process (BP) regulation of purine nucleotide metabolic process 0.0004461 Informative Direct
Biological Process (BP) negative regulation of cell communication 0.005844 Informative Inherited
Biological Process (BP) negative regulation of signaling 0.005668 Informative Inherited
Biological Process (BP) negative regulation of response to stimulus 0.004047 Informative Inherited
Biological Process (BP) protein localization to organelle 0.05044 Informative Inherited
Biological Process (BP) cellular component movement 0.01131 Informative Inherited
Biological Process (BP) locomotion 0.01035 Informative Inherited
Biological Process (BP) localization of cell 0.01396 Informative Inherited
Biological Process (BP) regulation of transferase activity 0.3763 Informative Inherited
Biological Process (BP) positive regulation of cell death 0.001467 Informative Inherited
Biological Process (BP) regulation of apoptotic process 1 Informative Inherited
Biological Process (BP) negative regulation of catalytic activity 0.00626 Informative Inherited
Biological Process (BP) regulation of cellular catabolic process 0.008768 Informative Inherited
Biological Process (BP) multicellular organismal reproductive process 0.007539 Informative Inherited
Biological Process (BP) single organism reproductive process 0.008491 Informative Inherited
Biological Process (BP) regulation of protein phosphorylation 0.2744 Informative Inherited
Biological Process (BP) negative regulation of protein phosphorylation 0.000003593 Highly Informative Direct
Biological Process (BP) protein folding 0 Highly Informative Direct
Biological Process (BP) spermatogenesis 0.00001158 Highly Informative Direct
Biological Process (BP) negative regulation of transferase activity 0.000002014 Highly Informative Direct
Biological Process (BP) protein localization to mitochondrion 0.00000000007276 Highly Informative Direct
Biological Process (BP) negative regulation of intracellular signal transduction 0.00004458 Highly Informative Direct
Molecular Function (MF) binding 0.09331 Least Informative Inherited
Molecular Function (MF) enzyme regulator activity 0.00009925 Moderately Informative Direct
Molecular Function (MF) unfolded protein binding 0 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.000001144 Least Informative Direct
Cellular Component (CC) membrane 0.03315 Least Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.02148 Least Informative Inherited
Cellular Component (CC) intracellular organelle part 0.7199 Least Informative Inherited
Cellular Component (CC) plastid 0.0000001227 Informative Direct

Document: GO annotation of SCOP domains

Worm Phenotype (WP)

(show details) Document: WP annotation of SCOP domains

Yeast Phenotype (YP)

(show details)
YP termFDR (all)SDYP levelAnnotation (direct or inherited)
Yeast Phenotype (YP)metabolism and growth0Least InformativeDirect

Document: YP annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)alimentary system0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)male organism0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)viscus0Least InformativeDirect
Xenopus ANatomical entity (XAN)portion of tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)gonad0Least InformativeDirect
Xenopus ANatomical entity (XAN)musculoskeletal system0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)ovary0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)endomesoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)lung0InformativeDirect
Xenopus ANatomical entity (XAN)skeletal system0InformativeDirect
Xenopus ANatomical entity (XAN)bone tissue0Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant ANatomical entity (PAN)guard cell0Least InformativeDirect
Plant structure DEvelopment stage (PDE)E expanded cotyledon stage0Least InformativeDirect
Plant structure DEvelopment stage (PDE)F mature embryo stage0Least InformativeDirect
Plant structure DEvelopment stage (PDE)D bilateral stage0Least InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processDNA replication0InformativeDirect
Biological processStress response0InformativeDirect
Cellular componentCytoplasm0Least InformativeDirect
Cellular componentEndoplasmic reticulum0.9163Moderately InformativeInherited
Cellular componentMicrosome0.0004096Highly InformativeDirect
DomainRepeat0Least InformativeDirect
DomainZinc-finger0Moderately InformativeDirect
Molecular functionMetal-binding0Least InformativeDirect
Molecular functionZinc0Least InformativeDirect
Post-translational modificationChaperone0InformativeDirect
Post-translational modificationMethylation0.00000008552Moderately InformativeDirect
Post-translational modificationPrenylation0InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR001305 SSF57938 Protein matches
Abstract

Molecular chaperones are a diverse family of proteins that function to protect proteins in the intracellular milieu from irreversible aggregation during synthesis and in times of cellular stress. The bacterial molecular chaperone DnaK is an enzyme that couples cycles of ATP binding, hydrolysis, and ADP release by an N-terminal ATP-hydrolyzing domain to cycles of sequestration and release of unfolded proteins by a C-terminal substrate binding domain. Dimeric GrpE is the co-chaperone for DnaK, and acts as a nucleotide exchange factor, stimulating the rate of ADP release 5000-fold [PubMed8016869]. DnaK is itself a weak ATPase; ATP hydrolysis by DnaK is stimulated by its interaction with another co-chaperone, DnaJ. Thus the co-chaperones DnaJ and GrpE are capable of tightly regulating the nucleotide-bound and substrate-bound state of DnaK in ways that are necessary for the normal housekeeping functions and stress-related functions of the DnaK molecular chaperone cycle.

Besides stimulating the ATPase activity of DnaK through its J-domain, DnaJ also associates with unfolded polypeptide chains and prevents their aggregation [PubMed15063739]. Thus, DnaK and DnaJ may bind to one and the same polypeptide chain to form a ternary complex. The formation of a ternary complex may result in cis-interaction of the J-domain of DnaJ with the ATPase domain of DnaK. An unfolded polypeptide may enter the chaperone cycle by associating first either with ATP-liganded DnaK or with DnaJ. DnaK interacts with both the backbone and side chains of a peptide substrate; it thus shows binding polarity and admits only L-peptide segments. In contrast, DnaJ has been shown to bind both L- and D-peptides and is assumed to interact only with the side chains of the substrate.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 2 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a DnaJ/Hsp40 cysteine-rich domain domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 2 hidden Markov models representing the DnaJ/Hsp40 cysteine-rich domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]