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Outer membrane phospholipase A (OMPLA) superfamily
SCOP classification
Superfamily statistics
Functional annotation
| General category | Metabolism |
| Detailed category | Lipid metabolism and transport |
Function annotation of SCOP domain superfamilies
InterPro annotation
| Cross references | IPR003187 SSF56931 Protein matches |
| Abstract | Outer membrane phospholipase A (OMPLA) is an integral membrane phospholipase, which is present in many
Gram-negative bacteria and has a broad substrate specificity . The role of OMPLA has been most thoroughly studied in Escherichia coli,
where it participates in the secretion of bacteriocins. Bacteriocin release is triggered by a lysis
protein (bacteriocin release protein or BRP), followed by a phospholipase dependent accumulation
of lysophospholipids and free fatty acids in the outer membrane [ 12615538]. The reaction products enhance the
permeability of the outer membrane, which allows the semispecific secretion of bacteriocins. One speculative function of OMPLA is related to organic solvent tolerance in bacteria. Structurally, it consists of a
12-stranded antiparallel beta-barrel with a convex and a flat side. The active site residues are exposed
on the exterior of the flat face of the beta-barrel. The activity of the enzyme is regulated by reversible
dimerisation. Dimer interactions occur exclusively in the
membrane-embedded parts of the flat side of the beta-barrel, with polar residues embedded in an
apolar environment forming the key interactions. The active site His and Ser residues are located at the exterior of the beta-barrel, at the outer
leaflet side of the membrane. This location indicates that under normal conditions the substrate and
the active site are physically separated, since in E. coli phospholipids are exclusively located in the
inner leaflet of the outer membrane. |
InterPro database
PDBeMotif information about ligands, sequence and structure motifs
PDBeMotif resource
Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation ]
Internal database links
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Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry
out SCOP domain assignments to all genomes at the superfamily level.
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Alignments of sequences to 1 models
in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical
are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.
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Browse and view proteins in genomes which have
different domain combinations including a Outer membrane phospholipase A (OMPLA) domain.
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Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.
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Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.
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There are 1 hidden Markov models representing the Outer membrane phospholipase A (OMPLA) superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.
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Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Internal database links ]
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