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  Home > SCOP hierarchy > Hect, E3 ligase catalytic domain superfamily


Hect, E3 ligase catalytic domain superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Alpha and beta proteins (a+b) [ 53931] (376)
Fold:   Hect, E3 ligase catalytic domain [ 56203]
Superfamily:   Hect, E3 ligase catalytic domain [ 56204]
Families:   Hect, E3 ligase catalytic domain [ 56205] (2)

Superfamily statistics
Genomes (442) Uniprot 2013_05 PDB chains (SCOP 1.75)
Domains 6,533 5,515 2
Proteins 6,385 5,443 2

 Functional annotation
General category Processes_IC
Detailed category Protein modification

Document: Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)protein metabolic process1.811e-080Least InformativeDirect
Biological Process (BP)cellular macromolecule metabolic process2.11e-070Least InformativeDirect
Biological Process (BP)developmental process5.748e-060.0002061Least InformativeDirect
Biological Process (BP)multicellular organismal process2.384e-050.0001005Least InformativeDirect
Biological Process (BP)regulation of metabolic process0.14160.1876Least InformativeInherited
Biological Process (BP)regulation of cellular process11Least InformativeInherited
Biological Process (BP)cellular protein modification process5.314e-115.513e-06Moderately InformativeDirect
Biological Process (BP)regulation of cellular metabolic process0.055260.2982Moderately InformativeInherited
Biological Process (BP)regulation of biosynthetic process0.22861Moderately InformativeInherited
Biological Process (BP)regulation of nitrogen compound metabolic process0.26070.282Moderately InformativeInherited
Biological Process (BP)single-organism developmental process1.974e-050.2337Moderately InformativeInherited
Biological Process (BP)anatomical structure morphogenesis0.076431Moderately InformativeInherited
Biological Process (BP)cellular catabolic process0.16230Moderately InformativeInherited
Biological Process (BP)organic substance catabolic process0.2390Moderately InformativeInherited
Biological Process (BP)nervous system development0.15930.2067Moderately InformativeInherited
Biological Process (BP)tissue development0.064630.8407Moderately InformativeInherited
Biological Process (BP)protein modification by small protein conjugation or removal00InformativeDirect
Biological Process (BP)tube development0.0078220.2048InformativeInherited
Biological Process (BP)anatomical structure formation involved in morphogenesis0.0035890.4414InformativeInherited
Biological Process (BP)cellular macromolecule catabolic process0.013190InformativeInherited
Biological Process (BP)tissue morphogenesis0.008070.5631InformativeInherited
Biological Process (BP)embryonic morphogenesis0.0074810.3539InformativeInherited
Biological Process (BP)protein catabolic process0.019610InformativeInherited
Biological Process (BP)proteolysis0.11990InformativeInherited
Biological Process (BP)chordate embryonic development1.082e-070.204InformativeInherited
Biological Process (BP)epithelium development0.13740.4316InformativeInherited
Biological Process (BP)protein ubiquitination00Highly InformativeDirect
Biological Process (BP)morphogenesis of embryonic epithelium5.053e-080.0003865Highly InformativeDirect
Biological Process (BP)modification-dependent protein catabolic process5.363e-060Highly InformativeDirect
Biological Process (BP)tube formation1.888e-050.0006213Highly InformativeDirect
Biological Process (BP)epithelial tube morphogenesis5.554e-070.007564Highly InformativeInherited
Molecular Function (MF)ligase activity, forming carbon-nitrogen bonds2.788e-141.712e-13InformativeDirect
Molecular Function (MF)small conjugating protein ligase activity2.587e-150Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) protein metabolic process 0 Least Informative Direct
Biological Process (BP) cellular macromolecule metabolic process 0 Least Informative Direct
Biological Process (BP) developmental process 0.0002061 Least Informative Direct
Biological Process (BP) multicellular organismal process 0.0001005 Least Informative Direct
Biological Process (BP) regulation of metabolic process 0.1876 Least Informative Inherited
Biological Process (BP) regulation of cellular process 1 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 0.4237 Least Informative Inherited
Biological Process (BP) localization 0.06122 Least Informative Inherited
Biological Process (BP) single-organism cellular process 0.9538 Least Informative Inherited
Biological Process (BP) response to stimulus 0.03464 Least Informative Inherited
Biological Process (BP) cellular protein modification process 5.513e-06 Moderately Informative Direct
Biological Process (BP) cellular catabolic process 0 Moderately Informative Direct
Biological Process (BP) organic substance catabolic process 0 Moderately Informative Direct
Biological Process (BP) regulation of protein metabolic process 3.288e-06 Moderately Informative Direct
Biological Process (BP) regulation of localization 1.602e-05 Moderately Informative Direct
Biological Process (BP) regulation of cellular component organization 0.0001426 Moderately Informative Direct
Biological Process (BP) macromolecule localization 0.0006111 Moderately Informative Direct
Biological Process (BP) regulation of cellular metabolic process 0.2982 Moderately Informative Inherited
Biological Process (BP) anatomical structure morphogenesis 1 Moderately Informative Inherited
Biological Process (BP) single-organism developmental process 0.2337 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.001784 Moderately Informative Inherited
Biological Process (BP) regulation of nitrogen compound metabolic process 0.282 Moderately Informative Inherited
Biological Process (BP) system process 0.3373 Moderately Informative Inherited
Biological Process (BP) positive regulation of metabolic process 0.5853 Moderately Informative Inherited
Biological Process (BP) regulation of biosynthetic process 1 Moderately Informative Inherited
Biological Process (BP) regulation of molecular function 0.1857 Moderately Informative Inherited
Biological Process (BP) regulation of gene expression 1 Moderately Informative Inherited
Biological Process (BP) organic substance transport 0.04353 Moderately Informative Inherited
Biological Process (BP) negative regulation of metabolic process 0.09557 Moderately Informative Inherited
Biological Process (BP) cellular localization 0.0726 Moderately Informative Inherited
Biological Process (BP) signal transduction 0.544 Moderately Informative Inherited
Biological Process (BP) regulation of multicellular organismal process 0.8434 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 1 Moderately Informative Inherited
Biological Process (BP) regulation of response to stimulus 0.001166 Moderately Informative Inherited
Biological Process (BP) regulation of cell communication 0.01374 Moderately Informative Inherited
Biological Process (BP) regulation of signaling 0.0295 Moderately Informative Inherited
Biological Process (BP) nervous system development 0.2067 Moderately Informative Inherited
Biological Process (BP) response to abiotic stimulus 0.8651 Moderately Informative Inherited
Biological Process (BP) reproduction 0.009746 Moderately Informative Inherited
Biological Process (BP) organ development 1 Moderately Informative Inherited
Biological Process (BP) organelle organization 0.7549 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.07992 Moderately Informative Inherited
Biological Process (BP) tissue development 0.8407 Moderately Informative Inherited
Biological Process (BP) protein modification by small protein conjugation or removal 0 Informative Direct
Biological Process (BP) cellular macromolecule catabolic process 0 Informative Direct
Biological Process (BP) protein catabolic process 0 Informative Direct
Biological Process (BP) proteolysis 0 Informative Direct
Biological Process (BP) negative regulation of response to stimulus 4.461e-06 Informative Direct
Biological Process (BP) regulation of ion transport 2.757e-05 Informative Direct
Biological Process (BP) response to DNA damage stimulus 4.045e-05 Informative Direct
Biological Process (BP) positive regulation of transport 0.0001803 Informative Direct
Biological Process (BP) negative regulation of cellular component organization 0.000679 Informative Direct
Biological Process (BP) blood circulation 0.0007569 Informative Direct
Biological Process (BP) negative regulation of cell communication 0.0008075 Informative Direct
Biological Process (BP) regulation of cellular component biogenesis 0.02121 Informative Inherited
Biological Process (BP) negative regulation of nitrogen compound metabolic process 0.003017 Informative Inherited
Biological Process (BP) regulation of response to stress 0.06104 Informative Inherited
Biological Process (BP) protein localization to organelle 0.005908 Informative Inherited
Biological Process (BP) growth 0.07911 Informative Inherited
Biological Process (BP) tube development 0.2048 Informative Inherited
Biological Process (BP) anatomical structure formation involved in morphogenesis 0.4414 Informative Inherited
Biological Process (BP) positive regulation of protein metabolic process 0.6193 Informative Inherited
Biological Process (BP) negative regulation of molecular function 0.1845 Informative Inherited
Biological Process (BP) multicellular organismal reproductive process 0.04305 Informative Inherited
Biological Process (BP) cellular process involved in reproduction 0.3333 Informative Inherited
Biological Process (BP) vesicle-mediated transport 0.6328 Informative Inherited
Biological Process (BP) negative regulation of cellular metabolic process 0.006064 Informative Inherited
Biological Process (BP) negative regulation of protein metabolic process 0.07409 Informative Inherited
Biological Process (BP) regulation of organelle organization 0.4662 Informative Inherited
Biological Process (BP) cytoplasmic transport 0.003101 Informative Inherited
Biological Process (BP) reproductive system development 0.02731 Informative Inherited
Biological Process (BP) gland development 0.0209 Informative Inherited
Biological Process (BP) developmental process involved in reproduction 0.5478 Informative Inherited
Biological Process (BP) regulation of cellular component movement 0.006331 Informative Inherited
Biological Process (BP) regulation of locomotion 0.01708 Informative Inherited
Biological Process (BP) positive regulation of signal transduction 0.6148 Informative Inherited
Biological Process (BP) tissue morphogenesis 0.5631 Informative Inherited
Biological Process (BP) embryonic morphogenesis 0.3539 Informative Inherited
Biological Process (BP) single organism reproductive process 0.01502 Informative Inherited
Biological Process (BP) sexual reproduction 0.008567 Informative Inherited
Biological Process (BP) enzyme linked receptor protein signaling pathway 0.05006 Informative Inherited
Biological Process (BP) cellular membrane organization 0.01278 Informative Inherited
Biological Process (BP) protein transport 0.006678 Informative Inherited
Biological Process (BP) chordate embryonic development 0.204 Informative Inherited
Biological Process (BP) epithelium development 0.4316 Informative Inherited
Biological Process (BP) protein ubiquitination 0 Highly Informative Direct
Biological Process (BP) morphogenesis of embryonic epithelium 0.0003865 Highly Informative Direct
Biological Process (BP) modification-dependent protein catabolic process 0 Highly Informative Direct
Biological Process (BP) tube formation 0.0006213 Highly Informative Direct
Biological Process (BP) regulation of transmembrane transport 7.642e-09 Highly Informative Direct
Biological Process (BP) endosomal transport 1.049e-08 Highly Informative Direct
Biological Process (BP) cellular membrane fusion 3.845e-08 Highly Informative Direct
Biological Process (BP) development of primary female sexual characteristics 6.794e-08 Highly Informative Direct
Biological Process (BP) negative regulation of sequence-specific DNA binding transcription factor activity 1.532e-07 Highly Informative Direct
Biological Process (BP) regulation of protein ubiquitination 3.677e-07 Highly Informative Direct
Biological Process (BP) regulation of cellular response to stress 1.195e-06 Highly Informative Direct
Biological Process (BP) regulation of protein catabolic process 1.764e-06 Highly Informative Direct
Biological Process (BP) ovulation cycle process 4.131e-06 Highly Informative Direct
Biological Process (BP) regulation of blood pressure 8.525e-05 Highly Informative Direct
Biological Process (BP) gonad development 0.0001232 Highly Informative Direct
Biological Process (BP) regulation of cellular response to growth factor stimulus 0.0001471 Highly Informative Direct
Biological Process (BP) regulation of sodium ion transport 0.0001554 Highly Informative Direct
Biological Process (BP) fertilization 0.0001565 Highly Informative Direct
Biological Process (BP) regulation of DNA metabolic process 0.0002316 Highly Informative Direct
Biological Process (BP) developmental growth 0.008906 Highly Informative Inherited
Biological Process (BP) negative regulation of protein modification process 0.001605 Highly Informative Inherited
Biological Process (BP) negative regulation of multicellular organismal process 0.04715 Highly Informative Inherited
Biological Process (BP) response to osmotic stress 0.01873 Highly Informative Inherited
Biological Process (BP) epithelial tube morphogenesis 0.007564 Highly Informative Inherited
Molecular Function (MF) binding 0.005859 Least Informative Inherited
Molecular Function (MF) protein binding 9.27e-07 Moderately Informative Direct
Molecular Function (MF) ligase activity, forming carbon-nitrogen bonds 1.712e-13 Informative Direct
Molecular Function (MF) enzyme binding 0.01063 Informative Inherited
Molecular Function (MF) small conjugating protein ligase activity 0 Highly Informative Direct
Molecular Function (MF) channel regulator activity 3.643e-12 Highly Informative Direct
Molecular Function (MF) transcription factor binding transcription factor activity 0.001 Highly Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.001157 Least Informative Inherited
Cellular Component (CC) intracellular organelle part 0.5208 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.7527 Least Informative Inherited
Cellular Component (CC) protein complex 0.8503 Least Informative Inherited
Cellular Component (CC) membrane 0.8496 Least Informative Inherited
Cellular Component (CC) endomembrane system 0.005747 Moderately Informative Inherited
Cellular Component (CC) organelle membrane 0.05185 Moderately Informative Inherited
Cellular Component (CC) Golgi apparatus 3.72e-06 Informative Direct
Cellular Component (CC) ubiquitin ligase complex 6.08e-06 Informative Direct
Cellular Component (CC) Golgi membrane 3.035e-07 Highly Informative Direct

Document: GO annotation of SCOP domains

Human Phenotype (HP)

(show details) Document: HP annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)growth variant0Least InformativeDirect
Worm Phenotype (WP)developmental timing variant0Least InformativeDirect
Worm Phenotype (WP)larval development variant0Least InformativeDirect
Worm Phenotype (WP)organism metabolism processing variant6.128e-05Least InformativeDirect
Worm Phenotype (WP)larval lethal0Moderately InformativeDirect
Worm Phenotype (WP)sterile progeny0Moderately InformativeDirect
Worm Phenotype (WP)protein aggregation variant1.2e-09Moderately InformativeDirect
Worm Phenotype (WP)pattern of transgene expression variant1.058e-07Moderately InformativeDirect
Worm Phenotype (WP)protein aggregation induced paralysis5.251e-06InformativeDirect

Document: WP annotation of SCOP domains

Yeast Phenotype (YP)

(show details) Document: YP annotation of SCOP domains

Fly Anatomy (FA)

(show details) Document: FA annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)male genitalia0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)gonad0Least InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant ANatomical entity (PAN)plant cell0Least InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processApoptosis4.636e-05Moderately InformativeDirect
Biological processDifferentiation0.0002824Moderately InformativeDirect
Biological processDNA damage0.0006129Moderately InformativeDirect
Biological processUbl conjugation pathway0InformativeDirect
Cellular componentNucleus1.269e-15Least InformativeDirect
Cellular componentCytoplasm0.0002786Least InformativeDirect
Cellular componentGolgi apparatus8.152e-07Moderately InformativeDirect
Cellular componentEndoplasmic reticulum0.000163Moderately InformativeDirect
DomainRepeat0Least InformativeDirect
DomainANK repeat3.583e-10InformativeDirect
Molecular functionMetal-binding1Least InformativeInherited
Molecular functionZinc-finger0.0007136Moderately InformativeDirect
Post-translational modificationLigase0Moderately InformativeDirect
Post-translational modificationPhosphoprotein4.447e-10Least InformativeDirect
Post-translational modificationUbl conjugation1.756e-15Moderately InformativeDirect

Document: KW annotation of SCOP domains

UniProtKB UniPathway (UP)

(show details)
UP termFDR (all)SDUP levelAnnotation (direct or inherited)
UniPathway (UP)biopolymer metabolism0Least InformativeDirect
UniPathway (UP)protein ubiquitination0Moderately InformativeDirect

Document: UP annotation of SCOP domains

InterPro annotation
Cross references IPR000569 SSF56204 Protein matches
Abstract The name HECT comes from 'Homologous to the E6-AP Carboxyl Terminus' [PubMed7708685]. Proteins containing this domain at the C-terminus include ubiquitin-protein ligase, which regulates ubiquitination of CDC25. Ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester, and then directly transfers the ubiquitin to targeted substrates. A cysteine residue is required for ubiquitin-thiolester formation. Human thyroid receptor interacting protein 12, which also contains this domain, is a component of an ATP-dependent multisubunit protein that interacts with the ligand binding domain of the thyroid hormone receptor. It could be an E3 ubiquitin-protein ligase. Human ubiquitin-protein ligase E3A interacts with the E6 protein of the cancer-associated Human papillomavirus type 16 and Human papillomavirus type 18. The E6/E6-AP complex binds to and targets the P53 tumor-suppressor protein for ubiquitin-mediated proteolysis.

InterPro database

PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Human Phenotype (HP) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Fly Anatomy (FA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · UniProtKB UniPathway (UP) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.

Alignments of sequences to 2 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a Hect, E3 ligase catalytic domain domain.

Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 2 hidden Markov models representing the Hect, E3 ligase catalytic domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Human Phenotype (HP) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Fly Anatomy (FA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · UniProtKB UniPathway (UP) · Internal database links ]
Please cite: Gough, J., Karplus, K., Hughey, R. and Chothia, C. (2001). "Assignment of Homology to Genome Sequences using a Library of Hidden Markov Models that Represent all Proteins of Known Structure." J. Mol. Biol., 313(4), 903-919.

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© Copyright 2013 The SUPERFAMILY authors and Julian Gough.