SUPERFAMILY 1.75 HMM library and genome assignments server


14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (496) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 2,831 3,511 8
Proteins 2,776 3,479 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)developmental process1.914e-080.0002558Least InformativeDirect
Biological Process (BP)single-organism cellular process6.955e-095.736e-06Least InformativeDirect
Biological Process (BP)localization1.803e-077.024e-07Least InformativeDirect
Biological Process (BP)response to stimulus0.038150.02932Least InformativeInherited
Biological Process (BP)multicellular organismal process9.625e-090.00707Least InformativeInherited
Biological Process (BP)regulation of metabolic process0.16220.5363Least InformativeInherited
Biological Process (BP)regulation of cellular process0.20380.8176Least InformativeInherited
Biological Process (BP)negative regulation of metabolic process0.00072940.0004533Moderately InformativeDirect
Biological Process (BP)cell differentiation7.491e-103.556e-05Moderately InformativeDirect
Biological Process (BP)macromolecule localization1.471e-107.109e-09Moderately InformativeDirect
Biological Process (BP)regulation of protein metabolic process0.00079232.249e-05Moderately InformativeDirect
Biological Process (BP)cellular localization6.149e-071.559e-08Moderately InformativeDirect
Biological Process (BP)organic substance transport2.745e-054.075e-06Moderately InformativeDirect
Biological Process (BP)regulation of localization0.28670.2409Moderately InformativeInherited
Biological Process (BP)positive regulation of cellular process0.81081Moderately InformativeInherited
Biological Process (BP)regulation of signal transduction0.2310.4329Moderately InformativeInherited
Biological Process (BP)regulation of cellular component organization0.13640.1075Moderately InformativeInherited
Biological Process (BP)signal transduction0.76030.5819Moderately InformativeInherited
Biological Process (BP)regulation of developmental process0.088020.6118Moderately InformativeInherited
Biological Process (BP)regulation of multicellular organismal process0.28580.8904Moderately InformativeInherited
Biological Process (BP)regulation of catalytic activity0.5080.02131Moderately InformativeInherited
Biological Process (BP)regulation of phosphate metabolic process14.461e-06Moderately InformativeInherited
Biological Process (BP)negative regulation of cellular process0.014840.007025Moderately InformativeInherited
Biological Process (BP)single-organism developmental process0.076980.3585Moderately InformativeInherited
Biological Process (BP)organ development0.24560.7134Moderately InformativeInherited
Biological Process (BP)nervous system development0.0012440.008674Moderately InformativeInherited
Biological Process (BP)protein transport06.977e-06InformativeDirect
Biological Process (BP)regulation of intracellular transport0.00030650.0002227InformativeDirect
Biological Process (BP)regulation of kinase activity4.067e-072.836e-05InformativeDirect
Biological Process (BP)generation of neurons7.55e-111.99e-05InformativeDirect
Biological Process (BP)negative regulation of protein metabolic process0.00087491.267e-06InformativeDirect
Biological Process (BP)positive regulation of cellular component organization0.0006020.0018InformativeInherited
Biological Process (BP)regulation of apoptotic process0.00077710.001723InformativeInherited
Biological Process (BP)regulation of organelle organization0.034750.01166InformativeInherited
Biological Process (BP)regulation of establishment of protein localization2.163e-070.3196InformativeInherited
Biological Process (BP)intracellular signal transduction0.046290.0008688InformativeInherited
Biological Process (BP)apoptotic process5.154e-050.001571InformativeInherited
Biological Process (BP)regulation of protein phosphorylation11InformativeInherited
Biological Process (BP)regulation of nervous system development0.036640.1079InformativeInherited
Biological Process (BP)regulation of cell development0.25120.378InformativeInherited
Biological Process (BP)central nervous system development0.00056330.004696InformativeInherited
Biological Process (BP)cellular component movement0.012960.1341InformativeInherited
Biological Process (BP)locomotion1.493e-050.007958InformativeInherited
Biological Process (BP)localization of cell0.010080.1673InformativeInherited
Biological Process (BP)positive regulation of organelle organization2.03e-050.0001508Highly InformativeDirect
Biological Process (BP)regulation of mitochondrion organization1.404e-091.303e-05Highly InformativeDirect
Biological Process (BP)telencephalon development3.057e-050.0001317Highly InformativeDirect
Biological Process (BP)negative regulation of protein modification process6.136e-051.015e-06Highly InformativeDirect
Biological Process (BP)regulation of cell division8.154e-051.991e-05Highly InformativeDirect
Biological Process (BP)intrinsic apoptotic signaling pathway3.295e-080.0005059Highly InformativeDirect
Molecular Function (MF)binding05.152e-12Least InformativeDirect
Molecular Function (MF)protein domain specific binding5.057e-141.15e-11Highly InformativeDirect
Cellular Component (CC)intracellular organelle part0.37270.7554Least InformativeInherited
Cellular Component (CC)membrane0.059690.231Least InformativeInherited
Cellular Component (CC)cytoplasmic part0.019570.0006259Least InformativeInherited
Cellular Component (CC)intracellular membrane-bounded organelle1.153e-080.009543Least InformativeInherited
Cellular Component (CC)vesicle0.0054240.007504Moderately InformativeInherited
Cellular Component (CC)organelle membrane0.0027110.005437Moderately InformativeInherited
Cellular Component (CC)cell junction1.417e-068.697e-05InformativeDirect
Cellular Component (CC)cytoplasmic vesicle membrane2.515e-050.0004171Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) developmental process 0.0002558 Least Informative Direct
Biological Process (BP) single-organism cellular process 5.736e-06 Least Informative Direct
Biological Process (BP) localization 7.024e-07 Least Informative Direct
Biological Process (BP) response to stimulus 0.02932 Least Informative Inherited
Biological Process (BP) multicellular organismal process 0.00707 Least Informative Inherited
Biological Process (BP) regulation of metabolic process 0.5363 Least Informative Inherited
Biological Process (BP) regulation of cellular process 0.8176 Least Informative Inherited
Biological Process (BP) negative regulation of metabolic process 0.0004533 Moderately Informative Direct
Biological Process (BP) regulation of phosphate metabolic process 4.461e-06 Moderately Informative Direct
Biological Process (BP) cell differentiation 3.556e-05 Moderately Informative Direct
Biological Process (BP) macromolecule localization 7.109e-09 Moderately Informative Direct
Biological Process (BP) regulation of protein metabolic process 2.249e-05 Moderately Informative Direct
Biological Process (BP) cellular localization 1.559e-08 Moderately Informative Direct
Biological Process (BP) organic substance transport 4.075e-06 Moderately Informative Direct
Biological Process (BP) regulation of localization 0.2409 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 1 Moderately Informative Inherited
Biological Process (BP) regulation of signal transduction 0.4329 Moderately Informative Inherited
Biological Process (BP) regulation of cellular component organization 0.1075 Moderately Informative Inherited
Biological Process (BP) regulation of developmental process 0.6118 Moderately Informative Inherited
Biological Process (BP) regulation of multicellular organismal process 0.8904 Moderately Informative Inherited
Biological Process (BP) regulation of catalytic activity 0.02131 Moderately Informative Inherited
Biological Process (BP) signal transduction 0.5819 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.007025 Moderately Informative Inherited
Biological Process (BP) single-organism developmental process 0.3585 Moderately Informative Inherited
Biological Process (BP) organ development 0.7134 Moderately Informative Inherited
Biological Process (BP) nervous system development 0.008674 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.013 Moderately Informative Inherited
Biological Process (BP) protein transport 6.977e-06 Informative Direct
Biological Process (BP) regulation of intracellular transport 0.0002227 Informative Direct
Biological Process (BP) intracellular signal transduction 0.0008688 Informative Direct
Biological Process (BP) regulation of kinase activity 2.836e-05 Informative Direct
Biological Process (BP) generation of neurons 1.99e-05 Informative Direct
Biological Process (BP) negative regulation of protein metabolic process 1.267e-06 Informative Direct
Biological Process (BP) positive regulation of cellular component organization 0.0018 Informative Inherited
Biological Process (BP) regulation of apoptotic process 0.001723 Informative Inherited
Biological Process (BP) regulation of organelle organization 0.01166 Informative Inherited
Biological Process (BP) regulation of establishment of protein localization 0.3196 Informative Inherited
Biological Process (BP) apoptotic process 0.001571 Informative Inherited
Biological Process (BP) regulation of protein phosphorylation 1 Informative Inherited
Biological Process (BP) regulation of nervous system development 0.1079 Informative Inherited
Biological Process (BP) regulation of cell development 0.378 Informative Inherited
Biological Process (BP) regulation of cell cycle process 0.3015 Informative Inherited
Biological Process (BP) central nervous system development 0.004696 Informative Inherited
Biological Process (BP) cellular component movement 0.1341 Informative Inherited
Biological Process (BP) locomotion 0.007958 Informative Inherited
Biological Process (BP) localization of cell 0.1673 Informative Inherited
Biological Process (BP) response to DNA damage stimulus 0.05012 Informative Inherited
Biological Process (BP) DNA damage checkpoint 9.682e-06 Highly Informative Direct
Biological Process (BP) positive regulation of organelle organization 0.0001508 Highly Informative Direct
Biological Process (BP) regulation of mitochondrion organization 1.303e-05 Highly Informative Direct
Biological Process (BP) telencephalon development 0.0001317 Highly Informative Direct
Biological Process (BP) negative regulation of protein modification process 1.015e-06 Highly Informative Direct
Biological Process (BP) regulation of cell division 1.991e-05 Highly Informative Direct
Biological Process (BP) intrinsic apoptotic signaling pathway 0.0005059 Highly Informative Direct
Molecular Function (MF) binding 5.152e-12 Least Informative Direct
Molecular Function (MF) enzyme regulator activity 0.001389 Moderately Informative Inherited
Molecular Function (MF) enzyme inhibitor activity 0.0006001 Informative Direct
Molecular Function (MF) protein domain specific binding 1.15e-11 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.0006259 Least Informative Direct
Cellular Component (CC) intracellular organelle part 0.7554 Least Informative Inherited
Cellular Component (CC) membrane 0.231 Least Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.009543 Least Informative Inherited
Cellular Component (CC) vesicle 0.007504 Moderately Informative Inherited
Cellular Component (CC) organelle membrane 0.005437 Moderately Informative Inherited
Cellular Component (CC) cell junction 8.697e-05 Informative Direct
Cellular Component (CC) cytoplasmic vesicle membrane 0.0004171 Highly Informative Direct

Document: GO annotation of SCOP domains

Disease Ontology (DO)

(show details)
DO termFDR (all)SDDO levelAnnotation (direct or inherited)
Disease Ontology (DO)organ system cancer0Least InformativeDirect
Disease Ontology (DO)nervous system cancer0Moderately InformativeDirect
Disease Ontology (DO)disease of mental health0.003381Moderately InformativeInherited
Disease Ontology (DO)meningioma0InformativeDirect
Disease Ontology (DO)schizophrenia6.079e-05InformativeDirect

Document: DO annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)central nervous system0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)viscus0Least InformativeDirect
Xenopus ANatomical entity (XAN)portion of tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)ectoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)surface structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)embryonic structure0.004055Moderately InformativeInherited
Xenopus ANatomical entity (XAN)skin0InformativeDirect
Xenopus ANatomical entity (XAN)marginal zone0.0002675Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant structure DEvelopment stage (PDE)seed development stage1.059e-07InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm6.214e-12Least InformativeDirect
Coding sequence diversityAlternative initiation7.745e-06Moderately InformativeDirect
Post-translational modificationAcetylation0Least InformativeDirect
Post-translational modificationPhosphoprotein1.009e-06Least InformativeDirect
Post-translational modificationNitration3.48e-09InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]